Development of a Novel Scintillation Proximity Competitive Hybridization Assay for the Determination of Phosphorothioate Antisense Oligonucleotide Plasma Concentrations in a Toxicokinetic Study

Development of an Ultrasensitive Noncomp

Development of an Ultrasensitive Noncompetitive Hybridization–Ligation Enzyme-Linked Immunosorbent Assay for the Determination of Phosphorothioate Oligodeoxynucleotide in Plasma

✍ Rosie Z. Yu; Brenda Baker; Alfred Chappell; Richard S. Geary; Ellen Cheung; Arth 📂 Article 📅 2002 🏛 Elsevier Science 🌐 English ⚖ 208 KB

An ultrasensitive noncompetitive hybridization-ligation heterogeneous enzyme-linked immunosorbent assay was developed for the quantitation of antisense phosphorothioate oligodeoxynucleotides in plasma using a 96-well plate format. The principle of the assay is based on heterogeneous noncompetitive b