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A specific method for the analysis of hydroxyproline in tissues and urine

✍ Scribed by Darwin J. Prockop; Sidney Udenfriend

Publisher
Elsevier Science
Year
1960
Tongue
English
Weight
669 KB
Volume
1
Category
Article
ISSN
0003-2697

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A modification of the method of hydroxyproline determination in proteins was devised. The modification consists of the hydrolysis of proteins in 72% perchloric acid of 100Β°C for 2 hr instead of 20 hr, autoclaving in 6 N HCl or 2 N Ba(OH)\*. Determination of hydroxyproline by the modified method does