A solid-phase immunoassay for the binding of cartilage proteoglycan to hyaluronic acid
β Scribed by Harold D. Keiser
- Book ID
- 107714403
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 515 KB
- Volume
- 160
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
The protein-keratan sulfate core of bovine nasal cartilage proteoglycan was purified by affinity chromatography on a column of immobilized hyaluronic acid. The hyaluronic acid was immobilized by reaction with a hydrazido-alkyl derivative of Sepharose in the presence of borohydride. Proteoglycan was
## Abstract Proteoglycan aggregates isolated from normal bovine knee cartilage were larger than those from osteoarthritic cartilage of the same joints and appeared relatively more resistant to digestion with leech hyaluronidase. Incubation of proteoglycan subunits from the arthritic cartilage with