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A simple, rapid, manual peptide microsequencing procedure

✍ Scribed by H.S. Lu; P.M. Yuan; J.M. Talent; R.W. Gracy

Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
440 KB
Volume
110
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method is described for manual Edman degradation at the nanomole level. The method is simple, requiring only two extraction steps which minimize extraction loss, time, and there is no need for a conversion step of the thiazohnones to the thiohydantoins. Two different back hydrolysis procedures are compared and their relative merits discussed. The procedure requires no specialized reagents or equipment other than an amino acid analyzer.

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