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A simple and rapid procedure for the direct detection of cytomegalovirus in urine samples

✍ Scribed by M. P. Landini; M. X. Guan; A. Ripalti; T. Lazzarotto; M. La Placa; B. Trevisani

Publisher: John Wiley and Sons
Year: 1990
Tongue: English
Weight: 358 KB
Volume: 4
Category: Article
ISSN: 0887-8013
DOI: 10.1002/jcla.1860040302

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✦ Synopsis

Abstract

We compared cytomegalovirus (CMV) isolation in tissue culture and viral DNA detection by DNA:DNA hybridization in 60 clinical urine samples concentrated by different procedures. Our results showed that urine concentration by filtration is the easiest and quickest procedure allowing further detection of CMV. With optimized working conditions, the CMV detection in urine can be done in 5 hr without requiring cell cultures or costly instruments.

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