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A simple quantitative HPLC assay for ifosfamide in biological fluids

โœ Scribed by Jennifer M. Margison; P. M. Wilkinson; T. Cerny; N. Thatcher

Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
253 KB
Volume
1
Category
Article
ISSN
0269-3879

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โœฆ Synopsis

A high performance liquid chromatography method is described for measuring Ifosfamide (I) in human serum. This involves solvent extraction, reverse phase HPLC and UV detection at 190 nm. Standard curves of peak height x detector sensitivity versus I concentration in serum were linear with a lower limit of detection of 100 nglml. Authentic C-labelled I cochromatographed with standard I and with I found in serum from treated patients. The concentrationtime curves of I determined by both HPLC and gas chromatography were indistinguishable. We conclude that this method is suitable for determining I pharmacokinetics in biological specimens.

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