A quantitative selected ion monitoring assay for ketoprofen determination in human biological fluids

✍ Scribed by J. De Graeve; C. Frankinet; J. E. Gielen

• Publisher: John Wiley and Sons
• Year: 1979
• Tongue: English
• Weight: 382 KB
• Volume: 6
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/bms.1200060605

✦ Synopsis

A new very sensitive method of ketoprofen [ (benzoyl-3-phenyl)2-propionic acid] determination in various biological fluids using selected ion monitoring is described. After ether extraction in acidic medium, ketoprofen and (benzoyl-4-phenyl)-2-butyric acid used as internal standard are methylated and their concentrations determined by monitoring the mlr values corresponding to their respective molecular ions. Standard deviation is below 5% at 100 ng ml-'. Detection limits range from 10 ng d-' for urine and serum samples to 50 ng ml-' for maternal milk specimens. This method has been applied successfully to various biological studies: pharmacokinetic profiles, bioavailability and transfer of the drug through physiological barriers.