HPLC assay for determination of amphotericin B in biological samples

✍ Scribed by R. Espada; J. M. Josa; S. Valdespina; M. A. Dea; M. P. Ballesteros; J. M. Alunda; J. J. Torrado

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 90 KB
• Volume: 22
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/bmc.947

✦ Synopsis

Abstract

A fast and selective HPLC method for assaying amphotericin B in biological samples was developed and validated. The chromatographic separation was achieved in less than 12 min on a reverse‐phase C~18~ column using an acetonitrile–acetic acid–water (52:4.3:43.7, v/v/v) mixture as mobile phase. The flow rate was 1 mL/min and the effluent was monitored at 406 nm. A linear response over the concentration range 0.1–10.0 µg/mL was obtained. Intra‐day and inter‐day RSDs were below 5% for all the sample types. This new HPLC method was applied to assay amphotericin B in plasma and several tissue samples such as kidney, liver, spleen and bone marrow. Application of this method to pharmacokinetic studies in mice and dog is provided. Copyright © 2007 John Wiley & Sons, Ltd.