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A simple method for the quantitation of isozyme patterns

✍ Scribed by Robert J. Klebe

Publisher
Springer
Year
1975
Tongue
English
Weight
421 KB
Volume
13
Category
Article
ISSN
0006-2928

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✦ Synopsis

Isozyme patterns may be analyzed quantitatively, without the use of a densitometer, by performing serial twofold dilutions of a sample to a visual end point. The specific activity (S) of a given dehydrogenase isozyme can be assessed in the presence of other isozymes catalyzing the same reaction, by (1) determining the isozyme titer (T) (defined as mg protein/ml in the last visible band) and (2) applying the formula S = K/T, where K is 1.6 X 10(-3) units/ml in the last visible band. The units/ml (U) in the starting material can be calculated from the equation U = K (2)n-1, where n is the number of the slot producing the last visible band.

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