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A simple method for the quantitative determination of muramic acid

✍ Scribed by O. Hadžija

Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
335 KB
Volume
60
Category
Article
ISSN
0003-2697

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✦ Synopsis

A simple method for microdetermination of muramic acid is elaborated. The method is based on the degradation of muramic acid to lactic acid, followed by degradation of the latter to acetaldehyde which can be determined calorimetrically with p-hydroxydiphenyl (PHD) A linear relationship exists between the concentration of muramic acid (up to 20 pg), and absorbance at 569 nm. Substances usually present in the hydrolysates of bacterial cell wall peptidoglycan do not interfere in the determination.

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New method for quantitative determinatio
New method for quantitative determination of uronic acids
✍ Nelly Blumenkrantz; Gustav Asboe-Hansen 📂 Article 📅 1973 🏛 Elsevier Science 🌐 English ⚖ 314 KB

A new method for determination of uronic acids with meta-hydroxydiphenyl is introduced. It is simpler, quicker, more sensitive, and more specific than other methods, and it needs lesser amounts of fluid. It is recommended for determination of acid mucopolysaccharides in biological materials.