A simple method for the quantitation of glycuronic acid-containing glycosaminoglycans with mucopolysaccharidases

✍ Scribed by Zensaku Yosizawa; Noboru Ototani; Shigeo Satake

• Publisher: Elsevier Science
• Year: 1983
• Tongue: English
• Weight: 538 KB
• Volume: 128
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(83)90373-1

✦ Synopsis

A simple method for the quantitative determination of glycuronic acid-containing glycosaminoglycans (UA-GAG) is described. Sample solutions of glycosaminoglycans were digested with chondroitinase AC, chondroitinase C, chondroitinase B, heparitinases, and Streptomyces hyaluronidase, respectively, and the absorbance was read at 232 nm after digestion, The contents of 4-O-sulfated N-acetylgalactosaminyl~( 1 -4)Dglucosiduronyl units (Ch-4S), 6-O-sulfated N-acetylgalactosaminly@( 1 -4)Dgfucosiduronyl units (Ch-6S) plus N-acetylgalactosaminylfi( 1 -4)D-ghrcosiduronyl units (Ch-OS), 4-U-sulfated N-acetylgalactosaminylS( 1 -4)~idosiduronyl units (D-4s) plus N-acetylgalactosaminyl@( 1 -4)L-idosiduronyl units (D-OS), heparan sulfate, and hyaluronic acid in the sample solutions were calculated from the absorbance with reference to that of the digestion products of known amounts of standard UA-GAG. The analytical data obtained with the mixtures of authentic UA-GAG were in close agreement with the theoretical values. Application of this procedure to the urinary GAG fractions from orthopedic patients gave satisfactory results.