A simple technique for the preparation a
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Dawn S. Luthe
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Article
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1983
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Elsevier Science
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English
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A method for preparing multiple sucrose gradients by quickly freezing layers of sucrose has been developed. These gradients may be stored in the freezer indefinitely, and thawed from 8 to 24 h at 4ยฐC before use. The middle region of the resulting sucrose gradients was linear. Thawing time and centri