The Multiskan spectrophotometric system and Coomassie brilliant blue G-250 protein-dye binding method have been used together to measure NaOH-solubilized protein in subcellular membrane fractions prepared from isolated rat adipose cells. Forty-eight samples can be read in duplicate within 1 min. Suc
β¦ LIBER β¦
A simple rapid method for determining sucrose concentration in sucrose gradient centrifugation
β Scribed by Rosenberg, M. ;Trop, M. ;Avtalion, R. ;Pinsky, A.
- Publisher
- Springer-Verlag
- Year
- 1971
- Weight
- 191 KB
- Volume
- 59
- Category
- Article
- ISSN
- 0344-838X
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## Abstract Rubellaβspecific IgM was measured in a single fraction of serum from a sucrose density gradient. Haemagglutination inhibition (HAI) tests were performed on paired aliquots of the fraction untreated and after treatment with 2βmercaptoethanol, dilutions of the aliquots being incubated ove