𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A simple colorimetric assay method for pyrophosphate in the presence of a 1000-fold excess of orthophosphate: Application of the method to the study of pyrophosphate metabolism in mitochondria

✍ Scribed by Svetlana E. Mansurova; Olga A. Deryabina

Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
525 KB
Volume
176
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

πŸ“œ SIMILAR VOLUMES

High-performance liquid chromatographic
High-performance liquid chromatographic determination of pyrophosphate in the presence of a 20,000-fold excess of orthophosphate
✍ Norimasa Yoza; Izumi Akazaki; Tetsuya Nakazato; Nobuyuki Ueda; Hiroki Kodama; Ak πŸ“‚ Article πŸ“… 1991 πŸ› Elsevier Science 🌐 English βš– 616 KB

An HPLC method was based on anion-exchange separation of pyrophosphate (diphosphate) and orthophosphate and postcolumn spectrophotometric detection at 140 degrees C with a molybdenum(V)-molybdenum(VI) reagent. The reagent was easy to prepare, stable for at least 6 months at room temperature, and rea

A modified colorimetric method for the d
A modified colorimetric method for the determination of orthophosphate in the presence of high ATP concentrations
✍ Pilar GonzΓ‘lez-Romo; Sobeida SΓ‘nchez-Nieto; Marina Gavilanes-RuΓ­z πŸ“‚ Article πŸ“… 1992 πŸ› Elsevier Science 🌐 English βš– 328 KB

We describe a modified colorimetric method that quantitates inorganic phosphate linearly up to 60 nmol, with high stability of the developed color and with a low interference by ATP concentration (up to 30 mM). This method is very suitable for use in ATPase enzymatic assays, especially with enzymes