A colorimetric method for the microdetermination of silicon in the presence of excess phosphorus
β Scribed by James H. Austin; Robert W. Rinehart Sr.; Eleanor Ball
- Publisher
- Elsevier Science
- Year
- 1972
- Tongue
- English
- Weight
- 362 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0026-265X
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The determination of phosphorus is one of the basic determinations in biochemical analyses, yet, when a simple, rapid and sensitive method was required for the determination of phosphorus in wheat lipids, standard existing methods were not found to be entirely satisfactory. These methods are based o
We describe a modified colorimetric method that quantitates inorganic phosphate linearly up to 60 nmol, with high stability of the developed color and with a low interference by ATP concentration (up to 30 mM). This method is very suitable for use in ATPase enzymatic assays, especially with enzymes