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A simple colorimetric assay for catechol-O-methyl transferase

✍ Scribed by W.F. Herblin

Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
208 KB
Volume
51
Category
Article
ISSN
0003-2697

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A rapid spectrophotometric assay for catechol-O-methyltransferase
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A new assay technique for catechol-0-methyltransferase is described. 3,4-Dihydroxyacetophenone is used as the substrate for the assay and the products, 3-hydroxy-4-methoxyacetophenone and 4-hydroxy-3-methoxyacetophenone are detected spectrophotometrically at 344 nm in borate buffer, pH 10.0. This sp

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## Abstract The synthesis of a series of compounds with the general structure shown in formula I is described. These compounds, especially 3, 4‐dihydroxyphenylacetamide (XVI), are potent and nontoxic inhibitors __in vivo__ of catechol‐O‐methyl transferase. They compare favourably with pyrogallol an