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A continuous spectrophotometric assay for catechol-O-methyltransferase

✍ Scribed by James K. Coward; Felicia Ying-Hsiueh Wu


Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
220 KB
Volume
55
Category
Article
ISSN
0003-2697

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A rapid spectrophotometric assay for cat
✍ Ronald T. Borchardt πŸ“‚ Article πŸ“… 1974 πŸ› Elsevier Science 🌐 English βš– 400 KB

A new assay technique for catechol-0-methyltransferase is described. 3,4-Dihydroxyacetophenone is used as the substrate for the assay and the products, 3-hydroxy-4-methoxyacetophenone and 4-hydroxy-3-methoxyacetophenone are detected spectrophotometrically at 344 nm in borate buffer, pH 10.0. This sp