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A simple and sensitive assay procedure for ribonucleotide reductase system

โœ Scribed by Yun-Chi Yeh

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
467 KB
Volume
86
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A sensitive, rapid, and accurate assay for measuring ribonucleotide reductase activity, including the reduction of CDP. UDP, GDP. and ADP to their corresponding deoxyribonucleotides, is described. Using polyethyleneiminecellulose thin-layer chromatography, I was able to separate the substrates, the reaction products, and the associated hydrolytic products from each other. This procedure allowed me to measure all of the products formed without dephosphorylation or acid hydrolysis. It has been adapted to study the ribonucleotide reductase system of bacteriophage TCinfected cells.

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