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A simple and sensitive assay for dopamine-β-hydroxylase activity by dual-wavelength spectrophotometry

✍ Scribed by Takeshi Kato; Hiroshi Kuzuya; Toshiharu Nagatsu

Book ID
115766283
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
519 KB
Volume
10
Category
Article
ISSN
0006-2944

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A simple and sensitive assay for dopamin
A simple and sensitive assay for dopamine-β-hydroxylase
✍ Tong Hyub Joh; Robert A. Ross; Donald J. Reis 📂 Article 📅 1974 🏛 Elsevier Science 🌐 English ⚖ 349 KB

A simple, rapid, and highly sensitive radiochemical assay for measuring the activity of dopamine+hydroxylase in tissues and serum is described. Enzyme activity is detected by converting [l-'"Cltyramine to [l-"Cloctopamine which is then subjected to periodate cleavage to form ["Clformamide. This radi