𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A simple and rapid chromatographic method for the immobilization of acetylcholinesterase from electric eel

✍ Scribed by R.M. Kothari; M.A. Grafius; D.B. Millar

Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
439 KB
Volume
71
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

Acetylcholinesterase from electric eel is selectively immobilized on Amberlite IR-120 resin equilibrated with Al 3+ ions. Immobilized acetylcholinesterase activity is stable at least for 85 days in the wet state at 10Β°C and for 180 days in the dry state at room temperature. Activity determinations in the presence of eserine sulfate, decamethonium bromide, quinidine sulfate and butyryl thiocholine iodide suggested that the immobilized enzyme exhibited essentially the same properties as did the free enzyme.

273

πŸ“œ SIMILAR VOLUMES

A simple and rapid method for purificati
A simple and rapid method for purification of profilin from bovine thyroid
✍ Ryoji Kobayashi; William A. Bradley; James B. Field πŸ“‚ Article πŸ“… 1982 πŸ› Elsevier Science 🌐 English βš– 390 KB

Profilin has been purified from bovine thyroid by a new and simple method, employing affinity chromatography on DNase I-agarose and gel filtration. This procedure should be particularly useful for purifying profilin from tissues in which it is present in relatively low concentration because it does

A simple and rapid method for the quanti
A simple and rapid method for the quantitative isolation of proteins from polyacrylamide gels
✍ M. Otto; Maja Ε nejdΓ‘rkovΓ‘ πŸ“‚ Article πŸ“… 1981 πŸ› Elsevier Science 🌐 English βš– 291 KB

A simple method for protein recovery from gel slices following polyacrylamide gel electrophoresis is proposed which requires neither additional devices nor much skill. The method is based on reversed electrophoresis using a discontinuous conductivity gradient which is in turn maintained by a gradien