A simple and general plant tissue extraction procedure for two-dimensional gel electrophoresis
✍ Scribed by Jorge E. Mayer; Günther Hahne; Klaus Palme; Jeff Schell
- Book ID
- 104694453
- Publisher
- Springer
- Year
- 1987
- Tongue
- English
- Weight
- 907 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0721-7714
No coin nor oath required. For personal study only.
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Three different extraction procedures for two-dimensional electrophoresis of plant proteins are compared: (i) extraction of soluble proteins with a nondenaturing Tris-buffer, (ii) denaturing extraction in presence of sodium dodecyl sulfate at elevated temperature allowing the solubilization of membr
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