✦ LIBER ✦

A series of novel directional cloning and expression vectors for blunt-end ligation of PCR products

✍ Scribed by Qiong Wu; Xing Zhong; Chao Zhai; Ju Yang; Xiaomei Chen; Liang Chen; Wenfeng Wang; Lixin Ma

Publisher: Springer Netherlands
Year: 2009
Tongue: English
Weight: 216 KB
Volume: 32
Category: Article
ISSN: 0141-5492
DOI: 10.1007/s10529-009-0166-3

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Twin PCRs: a simple and efficient method

Twin PCRs: a simple and efficient method for directional cloning of PCR products

✍ Bohong Xie; Yunfei Xie 📂 Article 📅 2011 🏛 Springer 🌐 English ⚖ 160 KB

pEGFP-T, a novel T-vector for the direct

pEGFP-T, a novel T-vector for the direct, unidirectional cloning and analysis of PCR-amplified promoters

✍ Baoli Wang; Xiaoxia Li; Fang Zheng; Rui Liu; Jinxing Quan; Hui Liang; Shanyi Guo 📂 Article 📅 2006 🏛 Springer Netherlands 🌐 English ⚖ 186 KB

Construction of a pTOC-T vector using GS

Construction of a pTOC-T vector using GST-ParE toxin for direct cloning and selection of PCR products

✍ Han Geun Kim; Hye Sun Kim; Hyun Jin Hwang; Sung Kyun Chung; Jung Min Lee; Dae Ky 📂 Article 📅 2004 🏛 Springer Netherlands 🌐 English ⚖ 147 KB

A hybrid promoter-containing vector for

A hybrid promoter-containing vector for direct cloning and enhanced expression of PCR-amplified ORFs in mammalian cells

✍ Lei-ming You; Jun Luo; Ai-ping Wang; Gai-ping Zhang; Hai-bo Weng; Ya-nan Guo; Yu 📂 Article 📅 2009 🏛 Springer 🌐 English ⚖ 580 KB

Construction of a series of vectors for

Construction of a series of vectors for high throughput cloning and expression screening of membrane proteins fromMycobacterium tuberculosis

✍ Huajun Qin; Jian Hu; Yuanzhi Hua; Shridhar V Challa; Timothy A Cross; Fei P Gao 📂 Article 📅 2008 🏛 BioMed Central 🌐 English ⚖ 899 KB

## Background One of the major challenges for membrane protein structural genomics is establishing high-throughput cloning and expression screening methods to obtain enough purified protein in a homogeneous preparation for structural and functional studies. Here a series of ligation independent clo