๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Twin PCRs: a simple and efficient method for directional cloning of PCR products

โœ Scribed by Bohong Xie; Yunfei Xie

Publisher
Springer
Year
2011
Tongue
English
Weight
160 KB
Volume
27
Category
Article
ISSN
1573-0972

No coin nor oath required. For personal study only.

๐Ÿ“œ SIMILAR VOLUMES

Comparative study of three methods for c
Comparative study of three methods for cloning PCR products
โœ Y. Abed; C. Bollet; P. Micco ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› Springer ๐ŸŒ English โš– 245 KB

The direct sequencing of the products of polymerase chain reactions (PCR) still presents difficulties and often requires special manipulations, such as the generation of excess single-stranded DNA using asymmetric PCR. Several alternative methods involve cloning PCR products into vector DNA suitable

A simple, efficient method for purificat
A simple, efficient method for purification of degraded PCR primers
โœ Andrea Fekete; John A. Bantle ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Springer-Verlag ๐ŸŒ English โš– 479 KB

## SUMMPARY A simple, efficient method was used to purify QCR primers which had degraded during storage at -2O"C/-9O'C. The primers were electrophoresed ou 3 A (w/v) agarose gel, the main band was electroeluted via a trough cut in the gel. The primers were recovered by isobutanol extraction follo