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A hybrid promoter-containing vector for direct cloning and enhanced expression of PCR-amplified ORFs in mammalian cells

โœ Scribed by Lei-ming You; Jun Luo; Ai-ping Wang; Gai-ping Zhang; Hai-bo Weng; Ya-nan Guo; Yun-chao Liu; Qiao-mu Li; Man Teng


Publisher
Springer
Year
2009
Tongue
English
Weight
580 KB
Volume
37
Category
Article
ISSN
0301-4851

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Most conditional expression vectors designed for mammalian cells have been valuable systems for studying genes of interest by regulating their expressions. The available vectors, however, are reliable for the short-length cDNA clones and not optimal for relatively long fragments of genomic DNA or lo