A method to quantitate collagen synthesis, total protein synthesis, and DNA in 24-well culture plates is presented. Collagen-producing cells such as human intestinal smooth muscle cells and dermal fibroblasts were pulse-labeled with [3H]proline. After incubation, the plates were heated to 90 degrees
β¦ LIBER β¦
A sensitive microassay for protein in cells cultured on collagen
β Scribed by L.J. Wallace; L.M. Partlow
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 532 KB
- Volume
- 87
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A microassay to quantitate collagen synt
β
R.F. Diegelmann; G.R. Bryson; L.C. Flood; M.F. Graham
π
Article
π
1990
π
Elsevier Science
π
English
β 627 KB
A Microassay for Measuring Glycogen in 9
β
M.J. GΓ³mez-LechΓ³n; X. Ponsoda; J.V. Castell
π
Article
π
1996
π
Elsevier Science
π
English
β 129 KB
Ethanol. A heat sensitizer on neuroblast
β
Bhola N. Rama; Kedar N. Prasad
π
Article
π
1986
π
John Wiley and Sons
π
English
β 516 KB
A simple, rapid, and sensitive method fo
β
Thomas E. Ferrari; Jack M. Widholm
π
Article
π
1973
π
Elsevier Science
π
English
β 390 KB
A simple, rapid, and sensitive method for the measurement of DNA, RNA, and protein synthesis in cell samples is presented. Tke method measures the amount of isotope incorporated into these macromolecules after cell collection on filter paper and washing with a methanol:chloroform:water mixture. Samp
A Combined Assay for DNA, Protein, and I
β
Charlotte Rehfeldt; Kathrin Walther
π
Article
π
1997
π
Elsevier Science
π
English
β 252 KB
Cytotoxic activity of antibodies to a co
β
A. Maoz; H. Dym; Sara Fuchs; M. Sela
π
Article
π
1973
π
John Wiley and Sons
π
English
β 589 KB