A Combined Assay for DNA, Protein, and Incorporated [3H] Label in Cultured Muscle Cells

A microtiter assay for determining prote

A microtiter assay for determining protein, acetylcholinesterase activity, and G418 (neomycin) resistance in cultured cells

✍ Hannah Friedman Elson; Mary K. Gentry; Bhupendra P. Doctor 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 483 KB

The Coomassie brilliant blue assay for the determination of protein has been extended to rapidly and conveniently measure the protein concentration of cells growing in culture in a 96-well microtiter format. Modifications of the standard assay include sodium hydroxide to solubilize the cells and ova