๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

A sensitive method for the detection of aspartate: 2-oxoglutarate aminotransferase activity on polyacrylamide gels

โœ Scribed by Toshiharu Yagi; Hiroyuki Kagamiyama; Mitsuhiro Nozaki

Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
813 KB
Volume
110
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

โœฆ Synopsis

A sensitive technique for the qualitative and semiquantitative determination of the activity of aspartate aminotransferase on polyacrylamide gels after electrophoresis is described. It relies on the ability of aspartate aminotransferase to produce SOS--in the transamination between L-cysteine sulfinate and 2-oxoglutarate. The method is based on the reduction of nitroblue tetrazolium by SOS--using phenazine methosulfate as a coupling agent. The method has been characterized using human and pig sera, crude homogenates and crystalline preparation from pig heart muscle, and bacterial crude extracts.

๐Ÿ“œ SIMILAR VOLUMES

A simple and sensitive staining method f
A simple and sensitive staining method for the detection of cellulase isozymes in polyacrylamide gels
โœ Raphael Goren; Moshe Huberman ๐Ÿ“‚ Article ๐Ÿ“… 1976 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 949 KB

A simple and rapid staining procedure is described for qualitative and quantitative determination of the activity of plant (Citrus sinensis (L.) Osbeck cv. Shamouti) andfungal (Trichodermata viride) cellulases in polyacrylamide gels. The method is based on the incorporation of carboxymethyl cellulos

A Permanent Zn2+ Reverse Staining Method
A Permanent Zn2+ Reverse Staining Method for the Detection and Quantification of Proteins in Polyacrylamide Gels
โœ M. Ferreras; J.G. Gavilanes; J.M. Garciasegura ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 660 KB

An optimized method for reverse staining of proteins in polyacrylamide gels is described. The method is based on the selective precipitation of a white imidazole-zinc complex all along the gel except in the protein bands. The high selectivity of this method allows a "toning procedure" which turns th

A method for the detection of urocanase
A method for the detection of urocanase on polyacrylamide gels and its use with crude cell extracts of Pseudomonas
โœ Michael V. Bell; Harold Hassall ๐Ÿ“‚ Article ๐Ÿ“… 1976 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 865 KB

A method is described for the specific detection of urocanase activity on polyacrylamide gels. It is dependent upon the reduction of nitro blue tetrazohum by the product of the urocanase reaction using phenazine methosulphate as a coupling agent. The method has been characterized using crude cell ex