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A simple and sensitive staining method for the detection of cellulase isozymes in polyacrylamide gels

✍ Scribed by Raphael Goren; Moshe Huberman

Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
949 KB
Volume
75
Category
Article
ISSN
0003-2697

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✦ Synopsis

A simple and rapid staining procedure is described for qualitative and quantitative determination of the activity of plant (Citrus sinensis (L.) Osbeck cv. Shamouti) andfungal (Trichodermata viride) cellulases in polyacrylamide gels. The method is based on the incorporation of carboxymethyl cellulose, a cellulase substrate, into the gels. After electrophoresis of crude extracts the gels are incubated in sodium-potassium phosphate buffer for the cellulase reaction which is stopped at the desired time by acidification of the gels in 60% sulfuric acid. The gels are then exposed to 2.0% KI + 0.2% Iz. No color develops in areas containing cellulase activity. The experimental procedure is described, and its different aspects are discussed.

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