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A method for the detection of urocanase on polyacrylamide gels and its use with crude cell extracts of Pseudomonas

✍ Scribed by Michael V. Bell; Harold Hassall

Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
865 KB
Volume
75
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method is described for the specific detection of urocanase activity on polyacrylamide gels. It is dependent upon the reduction of nitro blue tetrazohum by the product of the urocanase reaction using phenazine methosulphate as a coupling agent. The method has been characterized using crude cell extracts of Pseudomonas testosteroni and Pseudomonas putida.

After growth of the organisms in histidine-succinate medium each extract shows only one band of urocanase activity. The enzymes from the two species have significantly different electrophoretic mobilities.

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