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A rapid method for the detection of trypsinogen and chymotrypsinogen after electrophoretic separation of pancreas extract on polyacrylamide gel

โœ Scribed by J. Dijkhof; C. Poort


Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
165 KB
Volume
62
Category
Article
ISSN
0003-2697

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We describe a method for the detection of isoforms of several glycolytic enzymes by activity staining after native PAGE. The staining is based on coupled enzyme assays carried out on the gel after electrophoresis and is linked to the disappearance of NADH, which is visualized by fluorescence. This m