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A Sensitive Fluorometric Assay for 2′,3′-Cyclic Nucleotide 3′-Phosphohydrolase

✍ Scribed by Sulamith Weissbarth; Howard S. Maker; Gerard M. Lehrer; Steven Schneider; Murray B. Bornstein


Book ID
111170926
Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
261 KB
Volume
35
Category
Article
ISSN
0022-3042

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A new spectrophotometric assay for 2′,3′
✍ Charles E. Dreiling; Cynthia Mattson 📂 Article 📅 1980 🏛 Elsevier Science 🌐 English ⚖ 454 KB

A new continuous spectrophotometric method for determining 2',3'-cyclic nucleotide 3'-phosphohydrolase (EC 3.1.4.37) is described. The assay method involves monitoring the decrease in pH which accompanies the hydrolysis of 2',3'-cyclic AMP. The reaction is performed in the presence of phenol red and