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Fluorometric assay of 2′, 3′ -cyclic adenosine monophosphate 3′-phosphohydrolase with 1,N6-etheno-2-aza-adenosine 2′, 3′ -monophosphate

✍ Scribed by K. W. Lo; K. F. Yip; K. C. Tsou


Book ID
111169136
Publisher
John Wiley and Sons
Year
1975
Tongue
English
Weight
193 KB
Volume
25
Category
Article
ISSN
0022-3042

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1,N6-etheno-2-aza-adenosine 3′,5′-monoph
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I,N"-etheno-2-aza-adenosine 3',5'-monophosphate (cyclic Z-aza+AMP) has been shown to be a sensitive and an efficient substrate for the assay of cyclic-nucleotide phosphodiesterase. The relative activity is 75% compared to cyclic AMP. Two K, values of 503 and 15 pM were observed with the beef heart e

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## Abstract The specificity of the reported stimulation of protein synthesis affected by dibutyryl adenosine cyclic 3′,5′‐monophosphate in L5178Y cells was tested by assaying the effect of adenosine cyclic 2′,3′‐monophosphate. Protein synthesis was assayed as total acid precipitable ^3^H‐leucine. L