A RAPID ASSAY FOR 2′,3′-CYCLIC NUCLEOTIDE 3′-PHOSPHORYDROLASE
✍ Scribed by N. R. Sims; P. R. Carnegil
- Book ID
- 111169524
- Publisher
- John Wiley and Sons
- Year
- 1976
- Tongue
- English
- Weight
- 302 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0022-3042
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📜 SIMILAR VOLUMES
A new continuous spectrophotometric method for determining 2',3'-cyclic nucleotide 3'-phosphohydrolase (EC 3.1.4.37) is described. The assay method involves monitoring the decrease in pH which accompanies the hydrolysis of 2',3'-cyclic AMP. The reaction is performed in the presence of phenol red and
In the course of studies on cyclic nucleotide phosphodiesterase, the need for an assay which was both sensitive and continuous was realized. Most of the methods available for monitoring phosphodiesterase either depend on the use of accessory enzymes, and are accordingly subject to intrinsic limitati