𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A sensitive estimation of the percentage of guanine plus cytosine in deoxyribonucleic acid by high performance liquid chromatography

✍ Scribed by C.Y. Ko; J.L. Johnson; L.B. Barnett; H.M. McNair; J.R. Vercellotti

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
481 KB
Volume
80
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

A rapid high performance liquid chromatographic (HPLC) method is described which offers a fast quantitative procedure for determining the percentage of guanine (G) plus cytosine (C) in DNA preparations after acid hydrolysis in 88% formic acid. As little as 10 Fg of DNA is amenable to this analysis. Although thymine is sometimes poorly resolved from impurities at the solvent front, the percentage of G f C was found to be accurateiy determined using adenine (A) simply from the ratio of concentrations from C/(A + C) or (G + C)/(2 A + G + C). The HPLC method gave values for the percentage of G + C in close agreement with thermal melting point and buoyant density values determined for the DNA of ten bacterial species and two fungi.

πŸ“œ SIMILAR VOLUMES

Analysis of 7-methylguanine in deoxyribo
Analysis of 7-methylguanine in deoxyribonucleic acids by high-performance liquid chromatography
✍ Hidetaka Yuki; Takehiko Yajima; Hideki Kawasaki; Akira Yamaji πŸ“‚ Article πŸ“… 1979 πŸ› Elsevier Science 🌐 English βš– 438 KB

Conditions for the analysis of 7-methylguanine (7-MG) in the presence of major nucleic acid bases and other minor bases by means of high-performance liquid chromatography (hplc) were established. Purine bases were obtained by Chargaffs method to yield apurinic acid and were analyzed by hplc with str

Rapid Quantitative Determination of Sial
Rapid Quantitative Determination of Sialic Acids in Glycoproteins by High-Performance Liquid Chromatography with a Sensitive Fluorescence Detection
✍ K.R. Anumula πŸ“‚ Article πŸ“… 1995 πŸ› Elsevier Science 🌐 English βš– 563 KB

Sialic acids were specifically labeled with o-phenylenediamine-2HCl (OPD) to yield stable fluorescent quinoxaline derivatives. The sialic acids were released from the glycoprotein in a NaHSO4 solution (0.25 M 80 degrees C, 20 min) and derivatized in the same solution with the OPD (10 mg/ml (final co

Advances in the analysis of amino acid p
Advances in the analysis of amino acid phenylthiohydantoins by high performance liquid chromatography
✍ Carl L. Zimmerman; Ettore Appella; John J. Pisano πŸ“‚ Article πŸ“… 1976 πŸ› Elsevier Science 🌐 English βš– 437 KB

All the organic soluble amino acid phenylthiohydantoins are eluted (but not totally resolved) in under 40 min on either a Zorbax ODS or Permaphase ETH column. The superior resolution of the Zorbax ODS column and the complimentary characteristic of the Permaphase ETH column allow the isocratic analys