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Analysis of 7-methylguanine in deoxyribonucleic acids by high-performance liquid chromatography

✍ Scribed by Hidetaka Yuki; Takehiko Yajima; Hideki Kawasaki; Akira Yamaji

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
438 KB
Volume
97
Category
Article
ISSN
0003-2697

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✦ Synopsis

Conditions for the analysis of 7-methylguanine (7-MG) in the presence of major nucleic acid bases and other minor bases by means of high-performance liquid chromatography (hplc) were established. Purine bases were obtained by Chargaffs method to yield apurinic acid and were analyzed by hplc with strong cation-exchange resin, Zipax SCX. The method was applied to the determination of 7-MCI in the liver DNA from rats treated with DMN and it was revealed that the results were comparable to those obtained by radioisotope method in terms of sensitivity and reproducibility. The lower limits of quantitation and detection of 7-MC were determined to be 10 and 4 ng, respectively.

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