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A Sensitive, Continuously Recording Fluorogenic Assay for Calpain

✍ Scribed by Satish K. Mallya; Sheryl Meyer; Donna Bozyczko–Coyne; Robert Siman; Mark A. Ator

Book ID
115582374
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
76 KB
Volume
248
Category
Article
ISSN
0006-291X

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Sensitive fluorogenic substrates for trypsin, chymotrypsin, and elastase were prepared. These substrates are amides of an acyl amino acid or peptide with 7-amino-4-methyicoumarin (AMC). The substrates with their respective K,,,/K, ratios given in parentheses are: for chymotrypsin, glutaryl-Phe-AMC (