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A sensitive assay of sialidase activity with fluorogenic oligosaccharide derivatives

✍ Scribed by Masai Koseki; Michiko Ejiri; Shigeru Ino; Atsushi Kimura

Book ID
115767136
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
505 KB
Volume
29
Category
Article
ISSN
0006-2944

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This paper describes a new, sensitive, simple, rapid, and inexpensive assay of starch synthase using pyruvate kinase and phosphoenolpyruvate to convert the reaction product, ADP, to ATP, which is then quantified by use of the luciferin-luciferase system. The assay procedure covers the picomole to na