## Abstract We have designed and constructed an inexpensive imaging system based on charge coupled device (CCD) technology and utilized it to demonstrate the sensitivity and rapid detection possible with Lumigen® chemiluminescent reagents. We also report the development of two new chemiluminescent
A sensitive CCD image system for detection of chemiluminescent reactions
✍ Scribed by Jansen, Eugène H. J. M. ;Buskens, Carin A. F. ;van den Berg, Rijk H.
- Publisher
- John Wiley and Sons
- Year
- 1989
- Weight
- 397 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0884-3996
No coin nor oath required. For personal study only.
✦ Synopsis
A new ultra-sensitive light detection system is described for the detection of chemiluminescence reactions. The system consists of a CCD camera equipped with a two-stage image intensifier coupled to an image analysis system with real-time filtering capabilities. The sensitivity was determined with the enhanced chemiluminescence system using horseradish peroxidase as label. Additional possibilities for further increase in sensitivity and the potential use in immunoassays and other applications are discussed.
📜 SIMILAR VOLUMES
A sensitive and convenient detection system for 2-furancarboxylic acid (2-FA) was developed using a three-channel continuous-flow manifold with chemiluminescence (CL) detection. The CL reaction involves hydrogen peroxide, alkali, alcohol and 2-FA methyl ester. 2-FA is first esterified to methyl 23ur