Convenient and sensitive chemiluminescent detection system for 2-furancarboxylic acid using a continuous-flow method

✍ Scribed by Mikita Ishii; Kyoichi Itoh; Yoshiro Yoshihiro; Toshihiro Nakamura

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 810 KB
• Volume: 299
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(94)00337-8

✦ Synopsis

A sensitive and convenient detection system for 2-furancarboxylic acid (2-FA) was developed using a three-channel continuous-flow manifold with chemiluminescence (CL) detection. The CL reaction involves hydrogen peroxide, alkali, alcohol and 2-FA methyl ester. 2-FA is first esterified to methyl 23urancarboxylate

(2-FM) with 70% efficiency. Analytical figures of merit for 2-FM were as follows: detection limit, 1.0 X 1Qn6 M (signal-to-noise ratio = 2): determination range, 1.0 X 1O-6-O.1 M; sampling frequency, 60 h-l; and reproducibility (n = IO), 4.1% R.S.D. for I X IOF M Z-FM. A correlation between the present method and a gas ~hr~rna~o~a~hi~ method as a standard was good with a correlation coefficient of 0.999 (n = 5). The selectivity towards 2-FA compared with the related species, e.g., fury1 methyl ketone, was also good. The recovery for normal human sera was 97-110%.