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CCD camera imaging for the chemiluminescent detection of enzymes using new ultrasensitive reagents

✍ Scribed by Akhavan-Tafti, Hashem ;Schaap, A. Paul ;Arghavani, Zahara ;Desilva, Renuka ;Eickholt, Robert A. ;Handley, Richard S. ;Schoenfelner, Barry A. ;Sugioka, Katsuaki ;Sugioka, Yumiko

Publisher
John Wiley and Sons
Year
1994
Weight
703 KB
Volume
9
Category
Article
ISSN
0884-3996

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✦ Synopsis

Abstract

We have designed and constructed an inexpensive imaging system based on charge coupled device (CCD) technology and utilized it to demonstrate the sensitivity and rapid detection possible with Lumigen® chemiluminescent reagents. We also report the development of two new chemiluminescent reagents, Lumi‐Phos® Plus and Lumigen PS. Lumi‐Phos Plus is an ehanced formulation for the rapid detection of alkaline phosphatase on membranes and in solution. It provides excellent images in blotting applications with exposures of under a minute. Lumigen PS represents a new generation of peroxidase detection reagents. The wide dynamic range with excellent linearity, higher signal and lower background than other chemiluminescent reagents make Lumigen PS of unsurpassed value in enzyme‐linked immunoassays and nuclic acid probe assays using HRP conjugates.

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