The bioluminescent assay of ATP is rapid and simple and may be used as an estimate of microbial numbers. It therefore shows great potential as a technique t o provide information on the microbiological quality of a food within a few minutes, in comparison with conventional techniques, which provide
A review of bioluminescent ATP techniques in rapid microbiology
โ Scribed by Stanley, Philip E.
- Publisher
- John Wiley and Sons
- Year
- 1989
- Weight
- 466 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0884-3996
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A bioluminescent assay of bacterial ATP for rapid detection of bacterial growth in 512 clinical aerobic blood cultures was evaluated. At the detection limit of bacterial ATP (lO-'Omol/l) in the blood cultures 94.2% of the true positive blood cultures were detected (sensitivity) and the specificity w
ATP-sulfurylase (EC 2.7.7.4) catalyzes the first step in assimilatory sulfate reduction, forming adenosine 5'-phosphosulfate (APS) and pyrophosphate from ATP and SO:-. The extractable activity of ATP-sulfurylase was determined in crude extracts from Phaseolus vulgaris by measuring the formation of A
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