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A reliable general purpose method for extracting genomic DNA from Dictyostelium cells

✍ Scribed by Pilcher, Karen E; Fey, Petra; Gaudet, Pascale; Kowal, Anthony S; Chisholm, Rex L

Book ID
109946180
Publisher
Nature Publishing Group
Year
2007
Tongue
English
Weight
139 KB
Volume
2
Category
Article
ISSN
1750-2799

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✍ Awdhesh Kalia; Ashok Rattan; Prem Chopra πŸ“‚ Article πŸ“… 1999 πŸ› Elsevier Science 🌐 English βš– 143 KB

In this study, we report a modified procedure for extraction of high-quality genomic DNA that is rapid, simple, biologically nonhazardous, and generally applicable to pathogenic bacteria. Bacterial cells were pretreated with 70% ethanol prior to enzymatic digestion with lysozyme. Exposure of bacteri