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A robust universal method for extraction of genomic DNA from bacterial species

โœ Scribed by Sina Atashpaz; Sajjad Khani; Abolfazl Barzegari; Jaleh Barar; Sepideh Zununi Vahed; Reza Azarbaijani; Yadollah Omidi


Book ID
110172155
Publisher
SP MAIK Nauka/Interperiodica
Year
2010
Tongue
English
Weight
239 KB
Volume
79
Category
Article
ISSN
0026-2617

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A Method for Extraction of High-Quality
โœ Awdhesh Kalia; Ashok Rattan; Prem Chopra ๐Ÿ“‚ Article ๐Ÿ“… 1999 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 143 KB

In this study, we report a modified procedure for extraction of high-quality genomic DNA that is rapid, simple, biologically nonhazardous, and generally applicable to pathogenic bacteria. Bacterial cells were pretreated with 70% ethanol prior to enzymatic digestion with lysozyme. Exposure of bacteri