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A rapid spectrophotometric assay of phenylpyruvate oxidase activity

โœ Scribed by Brian L. Goodwin; Elizabeth G. Werner

Book ID
107711378
Publisher
Elsevier Science
Year
1972
Tongue
English
Weight
387 KB
Volume
50
Category
Article
ISSN
0003-2697

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New Spectrophotometric Assay for Polyphe
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โœ F. Gauillard; F. Richardforget; J. Nicolas ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 546 KB

We describe a convenient and sensitive assay of polyphenol oxidase (PPO, EC 1.14 .18 .1 ) consisting of spectrophotometry at \(300 \mathrm{~nm}\) based on the stoichiometric reaction of cysteine with o-quinones produced during the enzymatic oxidation of phenols. The adduct formed exhibited spectral