Spectrophotometric Assay of Ascorbic Acid Oxidase
โ Scribed by OBERBACHER, M. F.; VINES, H. M.
- Book ID
- 109625818
- Publisher
- Nature Publishing Group
- Year
- 1963
- Tongue
- English
- Weight
- 241 KB
- Volume
- 197
- Category
- Article
- ISSN
- 0028-0836
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L-Amino acid oxidase (LAO; 2 L-amino acid:O 2 oxidoreductase, EC 1.4.3.2) catalyzes the oxidative deamination of L-amino acids to produce the corresponding 2-oxo acids, hydrogen peroxide and ammonia. Many methods have been reported for the determination of LAO activity. They include measurement of o
A simple spectrophotometric method for estimation of diamine oxidase (DAO) activity was developed, based on a coupled reaction with NADH-dependent alcohol-dehydrogenase. Cystamine, upon DA0 action, is transformed into an aminoaldehyde which reacts quickly and quantitatively with NADH in the presenc