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A stopped spectrophotometric assay for the dopa oxidase activity of tyrosinase

✍ Scribed by Alison J. Winder

Book ID
116098244
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
588 KB
Volume
28
Category
Article
ISSN
0165-022X

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A simple spectrophotometric method for a rapid determination of tyrosinase (EC 1.14.18.1) is described. The basis of the assay is the incubation of the enzyme with L-dopa in the presence of an optimal concentration of Zn2+ ions and the measurement of the formation of melanochrome, as indicated by th