A sensitive and specific radioimmunoassay method for human erythrocyte carbonic anhydrase B was developed using a solid-phase technique. The assay was found to be sensitive for the detection of nanogram amounts of the enzyme in incubation mixtures. lodination of it gave rise to a loss of antigenicit
A rapid solid-phase radioimmunoassay for human luteinizing hormone
β Scribed by Margaret L. McKean; William R. Nes
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 372 KB
- Volume
- 73
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
β¦ Synopsis
A rapid and sensitive solid-phase radioimmunoassay for serum hLH has been developed which requires only 1 day to complete. Assay of sera from normal women provided values which agree with those obtained by other methods. At ovulation the mean of the hLH peaks for eight cycles was 27.1 mIU hLH/ml of serum. The mmimum was 1.7 mIU/ml in the luteal phase.
397
CopyrIght @ 1976 by Academic Pre5. Inc. All rights of reproductmn in any form rcwned.
π SIMILAR VOLUMES
A sensitive, reproducible, and rapid radioimmunoassay was developed to determine elastin peptide content of human serum in nanogram amounts. Significant differences have been determined between normal volunteers, both smokers and nonsmokers, and patients wth chronic obstructive pulmonary disease. Th
Antibodies directed against prostaglandin F\*CY and its main circulating metabolite, 13.14-dihydro-15-keto-prostaglandin F\*(Y. were insolubilized by reaction with ethylchloroformate. Suspensions of insolubilized antibodies retained immunogenic activity and were useful for the development of a simpl
A radioimmunoassay for the insulin receptor has been developed. In this assay, unlabeled receptor competes with 'ZSI-labeled receptor for binding to monoclonal anti-receptor antibodies immobilized on microtiter wells coated with affinity-purified anti-mouse immunoglobulin G. This assay was highly re
## Abstract A radioimmunoassay (RIA) for the detection of human IgG antibodies to cytomegalovirus (CMV) which utilizes extracts of CMVβinfected cells as antigen is described. Sera of 66 healthy adults were titered by the RIA and by the complement fixation (CF) test. These same subjects were also us