A rapid, solid phase radioimmunoassay for prostaglandin F2α and its main circulating metabolite

✍ Scribed by F.A. Fitzpatrick; M.A. Wynalda

• Publisher: Elsevier Science
• Year: 1976
• Tongue: English
• Weight: 579 KB
• Volume: 73
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(76)90155-x

✦ Synopsis

Antibodies directed against prostaglandin F*CY and its main circulating metabolite, 13.14-dihydro-15-keto-prostaglandin F*(Y. were insolubilized by reaction with ethylchloroformate. Suspensions of insolubilized antibodies retained immunogenic activity and were useful for the development of a simple and rapid solid phase radioimmunoassay for these two prostaglandins. Using this method, suppression of prostaglandin levels by the influenc: of indomethacin in Go has been observed. Advantages of solid phase radioimmunoassay over current procedures are discussed.

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Copyright Q 1976 hy Academic Pres. Inc. All right\ of reproduction in any form reerved.