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A solid-phase radioimmunoassay for human carbonic anhydrase B

✍ Scribed by Naoyuki Taniguchi; Takahito Kondo; Naoki Ishikawa; Hideki Ohno; Eimatsu Takakuwa; Ichiro Matsuda

Publisher: Elsevier Science
Year: 1976
Tongue: English
Weight: 552 KB
Volume: 72
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(76)90516-9

No coin nor oath required. For personal study only.

✦ Synopsis

A sensitive and specific radioimmunoassay method for human erythrocyte carbonic anhydrase B was developed using a solid-phase technique. The assay was found to be sensitive for the detection of nanogram amounts of the enzyme in incubation mixtures. lodination of it gave rise to a loss of antigenicity and a solid-phase assay technique using antibody-coated paper discs was employed in order to exclude the chemical modification or conformational changes of the enzyme. The levels of carbonic anhydrase B in the erythrocytes of newborn infants and in some biological fluids were determined.

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