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A rapid selective medium for the determination of trimethylamineoxide-reducing bacteria

✍ Scribed by J. M. Debevere; Prof. Dr. J. P. Voets

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
250 KB
Volume
14
Category
Article
ISSN
0233-111X

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✦ Synopsis

Abstract

A total of 50 Pseudomonas‐Achromobacter strains and 35 Enterobacteriaceae were tested on a selective medium for triamineoxidase activity. Stab inoculations of the organisms were made in a solid medium containing peptone, trimethylamineoxide‐hydrochloride (TMAO Β· HCl), optimum concentrations of Na and Mg for TMAO reduction and phenolred. Inoculated test tubes were incubated under anaerobic conditions. Positive results were obtained within 12‐18 h. Klebsiella spp., however, gave false negative results. In no case false positive results were obtained.

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